Partner study description
Samples were collected in Tiassale (5.898, -4.823), located in the evergreen forest zone of southern Côte d’Ivoire. The primary agricultural activity is rice cultivation in irrigated fields. High malaria transmission occurs during the rainy seasons, between May and November. Samples were collected as larvae from irrigated rice fields by dipping between May and September 2012. All larvae were reared to adults and females preserved over silica for DNA extraction. Specimens from this site were all An. coluzzii, determined by PCR assay (1).
See Grau-Bové et al. (2) for further details of this study.
1. F. Santolamazza, A. della Torre, and A. Caccone. Short report: a new polymerase chain reaction-restriction fragment length polymorphism method to identify anopheles arabiensis from an. gambiae and its two molecular forms from degraded dna templates or museum samples. The American Journal of Tropical Medicine and Hygiene, 70:604–6, July 2004. https://doi.org/10.4269/ajtmh.2004.70.604
2. Xavier Grau-Bové, Eric R Lucas, Dimitra Pipini, Emily Rippon, Arjèn van't Hof, Edi Constant, Samuel Dadzie, Alexander Egyir-Yawson, John Essandoh, Joseph Chabi, et al. Resistance to pirimiphos-methyl in west african anopheles is spreading via duplication and introgression of the ace1 locus. PLOS Genetics, 17(1):e1009253, January 2021. doi:10.1371/journal.pgen.1009253.
David Weetman (david.weetman [at] lstmed.ac.uk) Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
Edi Constant Centre Suisse de Recherches Scientifiques. Yopougon, Abidjan - 01 BP 1303 Abidjan, Côte d’Ivoire.