Partner study description
Specimens were obtained from two locations in Uganda: Nagongera, 30km to the North of Lake Victoria near the border with Kenya, and Kihihi, in the very South-West of the country. In Nagongera, Tororo District (0.77, 34.026), mosquitoes were collected by CDC light trap, resting and window trap collections, during October 2012. This is an area of intense perennial malaria transmission(1). Additional details of vector population bionomics may be found in (2, 3, 4, 5). Specimens were stored in 80% ethanol and DNA was extracted using the Qiagen plate protocol. In Kihihi subcounty, Kanungu District (-0.751, 29.701), resting mosquitoes were collected during October and November 2012. Kihihi is located in an upland area with seasonal malaria transmission (1). Specimens were stored in 80% ethanol and DNA was extracted using the Qiagen plate protocol. All specimens from both collections were An. gambiae (6).
For further details of this study see Kilama et al (1).
1. Maxwell Kilama, David L Smith, Robert Hutchinson, Ruth Kigozi, Adoke Yeka, Geoff Lavoy, Moses R Kamya, Sarah G Staedke, Martin J Donnelly, Chris Drakeley, Bryan Greenhouse, Grant Dorsey, and Steve W Lindsay. Estimating the annual entomological inoculation rate for plasmodium falciparum transmitted by anopheles gambiae s.l. using three sampling methods in three sites in uganda. Malaria J, March 2014. https://doi.org/10.1186/1475-2875-13-111
2. Paul Edward Okello, Wim Van Bortel, Anatol Maranda Byaruhanga, Anne Correwyn, Patricia Roelants, Ambrose Talisuna, Umberto D'Alessandro, and Marc Coosemans. Variation in malaria transmission intensity in seven sites throughout uganda. The American Journal of Tropical Medicine and Hygiene, 75:219–225, August 2006. https://doi.org/10.4269/ajtmh.2006.75.219
3. Urvashi Ramphul, Thomas Boase, Chris Bass, Loyce M. Okedi, Martin J. Donnelly, and Pie Müller. Insecticide resistance and its association with target-site mutations in natural populations of anopheles gambiae from eastern Uganda. Trans. R. Soc. Trop. Med. Hyg., 103:1121–1126, November 2009. https://doi.org/10.1016/j.trstmh.2009.02.014
4. Henry D. Mawejje, Craig S. Wilding, Emily J. Rippon, Angela Hughes, David Weetman, and Martin J. Donnelly. Insecticide resistance monitoring of field-collected anopheles gambiae s.l. populations from jinja, eastern uganda, identifies high levels of pyrethroid resistance. Medical and veterinary entomology, 27:276–283, September 2013. https://doi.org/10.1111/j.1365-2915.2012.01055.x
5. David Weetman, Keith Steen, Emily J Rippon, Henry D Mawejje, Martin J Donnelly, and Craig S Wilding. Contemporary gene flow between wild an. gambiae s.s. and an. arabiensis. Parasites & Vectors, July 2014. https://doi.org/10.1186/1756-3305-7-345
6. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x
Henry D. Mawejje Infectious Diseases Research Collaboration, 2C Nakasero Hill Road, PO Box 7475, Kampala, Uganda.
Martin J. Donnelly (Martin.Donnelly [at] lstmed.ac.uk) Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
David Weetman (david.weetman [at] lstmed.ac.uk) Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
Craig S. Wilding (C.S.Wilding [at] ljmu.ac.uk) School of Biological and Environmental Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK.