Partner study description
Pyrethrum spray collections were conducted in three villages in Cameroon during September and October 2009. These villages comprise a transect from forest (village of Mayos: 4.341, 13.558) to forest/savanna transition (village of Daiguene: 4.777, 13.844) to savanna (villages of Gado-Badzere and Zembe-Borongo: 5.747, 14.442) (1). All contributed specimens were An. gambiae s.s. (2). A proportion of specimens were karyotyped via scoring of polytene chromosomes (3). Specimens were stored on silica gel, and DNA was extracted using a simple CTAB protocol and run over Qiagen columns.
See Lobo et al. (1) for further details of this study
1. Neil F Lobo, Djibril M Sangaré, Allison A Regier, Kyanne R Reidenbach, David A Bretz, Maria V Sharakhova, Scott J Emrich, Sekou F Traore, Carlo Costantini, Nora J Besansky, and Frank H Collins. Breakpoint structure of the anopheles gambiae 2rb chromosomal inversion. Malaria J, 9:293, October 2010. https://doi.org/10.1186/1475-2875-9-293
2. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x
3. Mamadou B. Coulibaly, Marco Pombi, Beniamino Caputo, Davis Nwakanma, Musa Jawara, Lassana Konate, Ibrahima Dia, Abdrahamane Fofana, Marcia Kern, Frédéric Simard, David J. Conway, Vincenzo Petrarca, Alessandra della Torre, Sékou Traoré, and Nora J. Besansky. PCR-based karyotyping of Anopheles gambiae inversion 2Rj identifies the BAMAKO chromosomal form. Malaria J, 2007. https://doi.org/10.1186/1475-2875-6-133
Carlo Costantini (carlo.costantini [at] ird.fr) UMR MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier, France.
Kyanne R. Rohatgi Eck Institute for Global Health & Department of Biological Sciences, University of Notre Dame, IN 46556, USA.
Nora J. Besansky (nbesansk [at] nd.edu) Eck Institute for Global Health & Department of Biological Sciences, University of Notre Dame, IN 46556, USA.