Partner study description
Samples were contributed from pyrethrum spray collections, larval sampling and human landing catches conducted in twenty locations during October 2013. These villages are scattered throughout the country and reflect a gradient of human-dominated environments, for example, forest (Manda (5.726, 10.868) and Campo (2.367, 9.817)); forest/savanna transition (Tibati (6.469, 12.629)); savanna (Lagdo (9.049, 13.656)); suburban area (Nkolondom (3.972, 11.516)) and urban areas (Douala (4.055, 9.721) and Yaoundé (3.880, 11.506)). Contributed specimens were An. gambiae or An. coluzzii (1). Population genomics studies indicated the presence of relatively differentiated subgroups within both species as well as clusters thriving in polluted breeding sites in large cities (2). Specimens were stored on silica gel. DNA was extracted using a Zymo research kit for adults, and a Qiagen kit for larvae.
For further details of this study please see Kamdem et al (2).
1. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x
2. Colince Kamdem, Caroline Fouet, Stephanie Gamez, and Bradley J. White. Pollutants and Insecticides Drive Local Adaptation in African Malaria Mosquitoes. Mol. Biol. Evol., 34(5):1261–1275, February 2017. https://doi.org/10.1093/molbev/msx087
Colince Kamdem Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), B.P. 288, Yaoundé, Cameroon.
Caroline Fouet Department of Entomology, University of California, Riverside, CA, USA.
Bradley J. White (bradwhite [at] verily.com) Verily Life Sciences, South San Francisco, CA 94080, USA.