Partner study description
Collections were made from four different study sites around the border between Guinea and Mali. From Mali; Takan (11.47, -8.33) and Toumani Oulena (10.83, -7.81) are both small villages in the Yanfolila district of southern Mali and represent the Sudanian savannah ecological zone. Takan is arid savannah, while Toumani Oulena is humid savannah. In Guinea Conakry, mosquitoes were sampled from Koraboh (9.28, -10.03), a small village in the Kissidougou district in the Faranah region representing a semi-forest site with intermediate ecology, a mix of savannah and forest, and in Koundara (8.48, -9.53), a small village in the Macenta district in the Nzerekore region representing deep forest ecology. All reported collections occurred in October and November 2012. At each site, mosquitoes were collected using three different methods: human-landing capture, indoor manual aspirator or pyrethroid spray catch, and larval capture - where the first and second instar larvae were raised to adult in a field insectary under standard insectary conditions prior to DNA isolation from the adults, and the third and fourth instar larvae were preserved directly for DNA isolation, without rearing in the insectary. The two distinct methods of larval collection were used to control for possible genetic bias inherent in lab rearing of captured larvae. Across sites, all types of larval sites were sampled, including both temporary and permanent sites. Human-landing captures were performed both inside dwellings and outside (>10m from dwelling) at night between 18:00 and 06:30. The indoor aspirator or spray catches were done in the morning between 06:00 and 12:00. Adult specimens or third and fourth instar larvae were preserved immediately in 80% ethanol until later DNA extraction. First and second instar larvae were raised to adults in nearby field insectaries and upon emergence were preserved in 80% ethanol. DNA was extracted from mosquitoes using DNAzol by the provided protocol (Invitrogen, CA, USA).
See Boubacar et al. (1) for further details of this study.
1. Boubacar Coulibaly, Raymond Kone, Mamadou S. Barry, Becky Emerson, Mamadou B. Coulibaly, Oumou Niare, Abdoul H. Beavogui, Sekou F. Traore, Kenneth D. Vernick, and Michelle M. Riehle. Malaria vector populations across ecological zones in guinea conakry and mali, west africa. Malaria J, April 2016. doi:10.1186/s12936-016-1242-5.
Boubacar Coulibaly Malaria Research and Training Centre (MRTC), Faculty of Medicine and Dentistry, University of Mali, BP: E 423 Bamako, Mali.
Kenneth D. Vernick (kenneth.vernick [at] pasteur.fr) Unit for Genetics and Genomics of Insect Vectors, Institut Pasteur, 75015, Paris, France.
Michelle M. Riehle Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.