Partner study description
Mosquito samples were collected in Furvela (-23.716, 35.299), Mozambique, by CDC light traps between December 2003 and April 2004. Specimens were stored on silica gel and DNA was extracted according to (1). Contributed specimens consisted of An. gambiae individuals identified according to (2). Furvela is a rural village located in Inhambane Province, where malaria is transmitted mainly by An. gambiae and An. funestus (3). An. arabiensis and An. merus are also found at low frequency. Sporozoite rates around 4% have been reported in An. gambiae from Furvela (3).
1. Frank H. Collins, Nora J. Besansky, M. Alina Mendez, Melissa O. Rasmussen, Victoria Finnerty, and Philip C. Mehaffey. A ribosomal rna gene probe differentiates member species of the anopheles gambiae complex. The American Journal of Tropical Medicine and Hygiene, 37:37–41, July 1987. https://doi.org/10.4269/ajtmh.1987.37.37
2. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x
3. J. D. Charlwood, E. V. E. Tomas, N. Cuamba, and J. Pinto. Analysis of the sporozoite elisa for estimating infection rates in mozambican anophelines. Med Vet Entomol, 29:10–16, August 2014. https://doi.org/10.1111/mve.12084
João Pinto (jpinto [at] ihmt.unl.pt) Global Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Rua da Junqueira 100, 1349–008 Lisbon, Portugal.