Partner study description
Collections were performed during the rainy season in September 2002 by overnight CDC light traps in Sacriba of Bioko island (3.7, 8.7). Specimens were stored dry on silica gel before DNA extraction. Specimens contributed from this site were An. gambiae females, genotype determined by two assays (1, 2). These mosquitoes represent a population that inhabited Bioko Island before a comprehensive malaria control intervention initiated in February 2004 (3). After the intervention An. gambiae was declining, and more recently almost only An. coluzzii can be found (4).
1. Julie A. Scott, William G. Brogdon, and Frank H. Collins. Identification of single specimens of the anopheles gambiae complex by the polymerase chain reaction. The American Journal of Tropical Medicine and Hygiene, 49:520–529, October 1993. https://doi.org/10.4269/ajtmh.1993.49.520
2. F. Santolamazza, A. della Torre, and A. Caccone. Short report: a new polymerase chain reaction-restriction fragment length polymorphism method to identify anopheles arabiensis from an. gambiae and its two molecular forms from degraded dna templates or museum samples. The American journal of tropical medicine and hygiene, 70:604–6, July 2004. https://doi.org/10.4269/ajtmh.2004.70.604
3. Brian L Sharp, Frances C Ridl, Dayanandan Govender, Jaime Kuklinski, and Immo Kleinschmidt. Malaria vector control by indoor residual insecticide spraying on the tropical island of bioko, equatorial guinea. Malaria J, 6:52, 2007. https://doi.org/10.1186/1475-2875-6-52
4. Hans J Overgaard, Solve Sæbø, Michael R Reddy, Vamsi P Reddy, Simon Abaga, Abrahan Matias, and Michel A Slotman. Light traps fail to estimate reliable malaria mosquito biting rates on bioko island, equatorial guinea. Malaria J, February 2012. https://doi.org/10.1186/1475-2875-11-56
Jorge Cano London School of Hygiene and Tropical Medicine, Keppel Street, Bloomsbury, London WC1E 7HT, UK.
Maryam Kamali Department of Medical Entomology and Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA.
Igor Sharakhov (igor [at] vt.edu) Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA.
Department of Genetics and Cell Biology, Tomsk State University, Tomsk 634050, Russia.