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Ag1000G - MALI (AG1000G-ML-2)
Project: Ag1000G

Location: Mali (ML).

Mosquito

Partner study description

Collections of indoor resting adults were made by spray catch from seven villages in the southern part of Mali in August-September 2004: Banambani (12.800, -8.050), Bancoumana (12.200, -8.200), Douna (13.210, -5.900), Fanzana (13.200, -6.130), Kela (11.880, -8.450), Moribobougou (12.690, -7.870) and N’Gabakoro (12.680, -7.840). Specimens were sorted morphologically to An. gambiae s.l.. Ovaries of half-gravid females were dissected and placed in numbered individual micro-tubes containing modified Carnoy’s solution (1:3 glacial acetic acid: 100% ethanol). Carcasses were placed in correspondingly numbered micro-tubes over desiccant. Genomic DNA was isolated from individual mosquitoes using one of the following: DNeasy Extraction Kit (Qiagen, Valencia, CA), Puregene kit (Gentra Systems, Inc., Minneapolis, MN), DNAzol kit (Molecular Research Center, Inc., Cincinnati, OH.) or Easy-DNA kit (Invitrogen, Carlsbad, CA). An. gambiae s.s. and its molecular forms were identified using one of two rDNA-based PCR/RFLP assays (1, 2). Ovaries from specimens of the desired species were subject to polytene chromosome analysis.

For further details of this study see Mamadou et al (3).

1. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x

2. F. Santolamazza, A. della Torre, and A. Caccone. Short report: a new polymerase chain reaction-restriction fragment length polymorphism method to identify anopheles arabiensis from an. gambiae and its two molecular forms from degraded dna templates or museum samples. The American journal of tropical medicine and hygiene, 70:604–6, July 2004. https://doi.org/10.4269/ajtmh.2004.70.604

3. Mamadou B. Coulibaly, Marco Pombi, Beniamino Caputo, Davis Nwakanma, Musa Jawara, Lassana Konate, Ibrahima Dia, Abdrahamane Fofana, Marcia Kern, Frédéric Simard, David J. Conway, Vincenzo Petrarca, Alessandra della Torre, Sékou Traoré, and Nora J. Besansky. PCR-based karyotyping of Anopheles gambiae inversion 2Rj identifies the BAMAKO chromosomal form. Malaria J, 2007. https://doi.org/10.1186/1475-2875-6-133

Contributors

Mamadou B. Coulibaly Malaria Research and Training Centre (MRTC), Faculty of Medicine and Dentistry, University of Mali, BP: E 423 Bamako, Mali.

Moussa Keita Malaria Research and Training Centre (MRTC), Faculty of Medicine and Dentistry, University of Mali, BP: E 423 Bamako, Mali.

Cheick Traore Malaria Research and Training Centre (MRTC), Faculty of Medicine and Dentistry, University of Mali, BP: E 423 Bamako, Mali.

Nora J. Besansky (nbesansk@nd.edu) Eck Institute for Global Health & Department of Biological Sciences, University of Notre Dame, IN 46556, USA.