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Mosquito partner study resources

We cannot accept any samples until the below checklist is complete. If you have any queries regarding any aspect in relation to your partner study, please contact samples@malariagen.net, particularly if you need to request shipping kits, require support applying for Nagoya approval and to send your completed sample information sheet.

Checklist for shipping mosquito samples

  1. The study has a MalariaGEN Study ID
  2. Relevant ethics approvals are in place and have been shared with MalariaGEN
  3. Agreement from National authorities in the country of sample origin in line with the Nagoya Protocol is in place and signed off by Wellcome Sanger Institute
  4. Samples meet requirements and sampling/shipping protocols have been followed
  5. Two copies of the Sample Information Sheet (SIS) have been fully completed following sample instructions

1. Study ID
All MalariaGEN collaborations have a Study ID. We can only accept samples from approved partner studies. This should have been completed during study set-up.

2. Ethics approvals
All relevant ethics approvals for your study should be shared with MalariaGEN prior to receipt and processing of samples in line with good ethical practice. This should have been completed during study set-up, but please provide any updated approvals as they are agreed.

3. Nagoya approval
MalariaGEN cannot accept samples at Wellcome Sanger Institute without relevant Nagoya approval. This should have been completed during study set-up but the in-country process may have changed since and must be checked prior to each sample shipment. Full details can be found on the ABSCH Clearing House, but MalariaGEN are able to help with this process. Material Transfer Agreements are often a requirement, these should use your Institutional template so MalariaGEN does not provide a template as a resource. Wellcome Sanger Institute will need to sign off that due process has been followed.

Please note that approval is required from the country the samples originated in and acquiring approval can be a lengthy process that is best started as soon as possible.

4. Sample requirements
We can only accept whole mosquitoes that have been plated in ethanol. The best sequencing results come from mosquitoes that have been collected straight into ethanol, though previously desiccated mosquitoes that have been transferred into ethanol are also viable. We recommend transfer to ethanol as soon as possible. We may also accept extracted DNA with a minimum volume of 200ng in 100ul.

Species that we routinely process are:

  • Anopheles gambiae
  • Anopheles funestus

Download the collection shipping protocol

5. Sample Information Sheets (SIS)
We require two completed copies of this form: an electronic copy that must be emailed to samples@malariagen.net before shipping samples, and a printed copy that must be included within the sample shipment.

Each sample information sheet is an excel file that contains three tabs:

  1. An ‘Instructions’ tab, providing guidance on how to complete each sample metadata column
  2. An ‘Anopheles Samples’ tab that must be fully completed for each sample
  3. A ‘Location’ tab that must be fully completed for all samples

Download mosquito sample information sheet

Mosquito collection and storage
Collection shipping protocol

 

Sample information sheet (SIS)

Mosquito SIS

Mosquito SIS user guide