This page contains information about the Pf6 data release: data generated by the Plasmodium falciparum Community Project using the version 6 pipeline for variant discovery and genotype calling. This release contains sample information, accession numbers and genotype calls for samples as described in Plasmodium falciparum Community Project: about the version 6 data.
In 2018 the Plasmodium falciparum Community Project upgraded to version 6 of its variant discovery and genotype calling pipeline. Details of the methods can be found in the accompanying paper. The major change from previous versions is that the version 6 pipeline is based on GATK and utilises findings on genome accessibility generated by P. falciparum Genetic Crosses Project.
These data are available open access. Publications using these data should acknowledge and cite the source of the data using the following format: “This publication uses data from the MalariaGEN Plasmodium falciparum Community Project as described in ‘An open dataset of Plasmodium falciparum genome variation in 7,000 worldwide samples. MalariaGEN et al, Wellcome Open Research 2021642 DOI: 10.12688/wellcomeopenres.16168.1‘”.
Details of the 49 contributing partner studies, including description, contact information and key people.
Sample provenance and sequencing metadata
Sample information including partner study information, location and year of collection, ENA accession numbers, and QC information for 7,113 samples from 28 countries.
Measure of complexity of infections
Characterisation of within-host diversity (FWS) for 5,970 QC pass samples
Drug resistance marker genotypes
Genotypes at known markers of drug resistance for 7,113 samples, containing amino acid and copy number genotypes at six loci: crt, dhfr, dhps, mdr1, kelch13, plasmepsin 2-3.
Inferred resistance status classification
Classification of 5,970 QC pass samples into different types of resistance to 10 drugs or combinations of drugs and to RDT detection: chloroquine, pyrimethamine, sulfadoxine, mefloquine, artemisinin, piperaquine, sulfadoxine- pyrimethamine for treatment of uncomplicated malaria, sulfadoxine- pyrimethamine for intermittent preventive treatment in pregnancy, artesunate-mefloquine, dihydroartemisinin-piperaquine, hrp2 and hrp3 genes deletions.
Drug resistance markers to inferred resistance status
Details of the heuristics utilised to map genetic markers to resistance status classification.
Estimates of global and local differentiation for 5,561 genes.
Short variant genotypes
Genotype calls on 6,051,696 SNPs and short indels in 7,113 samples from 29 countries, available both as VCF and zarr files.
Download genotype call VCF: ftp://ngs.sanger.ac.uk/production/malaria/pfcommunityproject/Pf6/Pf_6_vcf/
Download genotype call zarr: ftp://ngs.sanger.ac.uk/production/malaria/pfcommunityproject/Pf6/Pf_6.zarr.zip
NOTE: You may need to download a free FTP client to access the FTP links.
Acknowledging and citing these data:18 Apr 2023
This release contains details on contributing partner studies, sample metadata and key sample attributes inferred from genomic data, and genomic data including raw sequence reads. Further details and analytical results can be found in the accompanying data release paper: An open dataset of Plasmodium falciparum genome variation in 7,000 worldwide samples. MalariaGEN et al, Wellcome Open Research 2021642 DOI: 10.12688/wellcomeopenres.16168.1
A README file describes in fine detail all the files included in the release, the format and interpretation of each column, and contains some tips and tricks for accessing genotype data in VCF and zarr files.
Data package contact
Publications using these data should acknowledge and cite the source of the data using the following format:
“This publication uses data from the MalariaGEN Plasmodium falciparum Community Project as described in ‘An open dataset of Plasmodium falciparum genome variation in 7,000 worldwide samples. MalariaGEN et al, Wellcome Open Research 2021642 DOI: 10.12688/wellcomeopenres.16168.1’”