1031 Artemisinin Resistance Confirmation, Characterization and Containment (ARC3)


Locations: Bangladesh (BD), Cambodia (KH), Thailand (TH)

Partner study description

ARC3, an international collaboration led by the World Health Organization, supported clinical trials of artesunate monotherapy in Cambodia, Thailand and Bangladesh. ARC3 aimed to confirm clinical resistance to artemisinin in South-East Asia, define in vitro drug resistance phenotypes, and identify molecular markers of resistance. One aspect of this work was developing methods to identify candidate markers of drug resistance using outputs from Illumina sequencing, laying the groundwork for large-scale studies to use whole genome sequencing and other high-throughput technologies to rapidly identify genetic loci associated with artemisinin resistance. This collaboration included investigating signatures of selection and genome-wide association studies of sensitive and resistant parasite isolates collected in the ARC3 trials, and resulted in improved handling and sequencing methods for field samples. Candidate markers discovered using this approach will be assessed for their ability to predict clinical drug resistance, and if validated, used to develop surveillance tools to guide containment efforts.

Key People

Dr Shannon Takala-Harrison
Center for Vaccine Development and Global Health, University of Maryland, School of Medicine, Baltimore, MD, USA
Mark Fukuda
Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences (USAMC-AFRIMS), Bangkok, Thailand
Prof Francois Nosten
Professor in Tropical Medicine, Group Head / PI, Consultant Physician and Fellow
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine Research building, University of Oxford Old Road campus, Oxford, UK
Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand
Harald Noedl
MARIB - Malaria Research Initiative Bandarban, Bangladesh
Medical University of Vienna, Vienna, Austria